The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
RelevanceDHRS2 belongs to the short-chain dehydrogenases/reductases (SDR) family. It may inhibit cell replication either by catalyzing the oxidation of estrogen and androgen or by converting cortisone in cortisol.
Cellular localizationMitochondrion matrix. Nucleus. Note: A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal.