Overview

  • Product nameAnti-DHRS7 antibody
    See all DHRS7 primary antibodies
  • Description
    Mouse polyclonal to DHRS7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length DHRS7 protein (Human) (NP_057113.1)

  • Positive control
    • DHRS7 transfected 293T cell lysate and human kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 36 and 37 kDa (predicted molecular weight: 38 kDa).
    Detection Antibody.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Information by UniProt
    • Database links
    • Alternative names
      • CGI 86 antibody
      • CGI-86 antibody
      • CGI86 antibody
      • Dehydrogenase/reductase (SDR family) member 7 antibody
      • Dehydrogenase/reductase SDR family member 7 antibody
      • Dhrs7 antibody
      • DHRS7_HUMAN antibody
      • DHRS7A antibody
      • retDSR4 antibody
      • Retinal short chain dehydrogenase/reductase 4 antibody
      • Retinal short-chain dehydrogenase/reductase 4 antibody
      • retSDR4 antibody
      • SDR34C1 antibody
      • Short chain dehydrogenase/reductase family 34C, member 1 antibody
      see all

    Anti-DHRS7 antibody images

    • Anti-DHRS7 antibody (ab69348) at 1/500 dilution + human kidney tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DHRS7 antibody (ab69348) at 1/500 dilution

      Lane 1 : DHRS7 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • IHC-P image of DHRS7 (ab69348) on Human Pancreas sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in H2O2 solution for 10 mins at 22°C.

      See Abreview

    References for Anti-DHRS7 antibody (ab69348)

    ab69348 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
    Permeabilization No
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3µg/mL · Temperature: 22°C
    Username

    Dr. Aamir Ahmed

    Verified customer

    Submitted Sep 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"