Overview

  • Product nameAnti-DHX15 antibody
    See all DHX15 primary antibodies
  • Description
    Rabbit polyclonal to DHX15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human DHX15 aa 650-700.
    Database link: 1665

Properties

Applications

Our Abpromise guarantee covers the use of ab13460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/30000. Predicted molecular weight: 95.4 kDa.

Target

Anti-DHX15 antibody images



  • Predicted band size : 95.4 kDa


    Detection of Human DHX 15 by Western Blot. Samples: Whole cell lysate (55 mcg/lane) from human lymphoblasts. Antibody: Affinity purified rabbit anti-DHX15 antibody 13460 used at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 1 and 5 minutes.
  • Anti-STAT1 antibody (ab26859) at 1/2000 dilution + STAT1 Ecoli derived fusion protein

    Secondary
    Goat anti IgY HRP at 1/1000 dilution

    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-DHX15 antibody (ab13460)

ab13460 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"