Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX15
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 750-795 of human DHX15 (Swiss-Prot O43143).

  • Positive control
    • 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP Use at 1-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-DHX15 antibody (ab70454) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells.
    Lane 2 : Whole cell lysate from 293T cells.

    Lysates/proteins at 60 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
  • 1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.

References

This product has been referenced in:
  • Chen YL  et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . Read more (PubMed: 24823796) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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