Overview

  • Product nameAnti-DHX15 antibody
    See all DHX15 primary antibodies
  • Description
    Rabbit polyclonal to DHX15
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 750-795 of human DHX15 (Swiss-Prot O43143).

  • Positive control
    • 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP Use at 1-5 µg/mg of lysate.

Target

Anti-DHX15 antibody images

  • All lanes : Anti-DHX15 antibody (ab70454) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells.
    Lane 2 : Whole cell lysate from 293T cells.

    Lysates/proteins at 60 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
  • 1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.

References for Anti-DHX15 antibody (ab70454)

This product has been referenced in:
  • Chen YL  et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . Read more (PubMed: 24823796) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70454.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"