Overview

  • Product name
    Anti-DHX16 antibody
  • Description
    Rabbit polyclonal to DHX16
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DHX16 aa 991-1041 (C terminal). The exact sequence is proprietary. NP_003578.1
    Sequence:

    LTTKEFMRQV LEIESSWLLE VAPHYYKAKE LEDPHAKKMP KKIGKTREEL G


    Database link: O60231

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 119 kDa.

Target

  • Function
    Probable ATP-binding RNA helicase involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410006N22Rik antibody
    • ATP-dependent RNA helicase #3 antibody
    • ATP-dependent RNA helicase 3 antibody
    • DAQB-47P19.12 antibody
    • DBP 2 antibody
    • DBP2 antibody
    • DDX 16 antibody
    • DDX16 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16 antibody
    • DEAD/H box 16 antibody
    • DEAH (Asp-Glu-Ala-His) box polypeptide 16 antibody
    • DEAH box protein antibody
    • DEAH box protein 16 antibody
    • DEAH-box protein 16 antibody
    • DEAH-box protein antibody
    • DHX16 antibody
    • DHX16_HUMAN antibody
    • fa91b12 antibody
    • KIAA0577 antibody
    • mKIAA0577 antibody
    • PRO2014 antibody
    • PRP 8 antibody
    • Prp2 antibody
    • PRP8 antibody
    • PRPF2 antibody
    • Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 antibody
    • RNA helicase antibody
    • wu:fa91b12 antibody
    • zgc:55590 antibody
    see all

Images

  • Detection of DHX16 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176819 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176819 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

  • All lanes : Anti-DHX16 antibody (ab176819) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 119 kDa


    Exposure time : 10 seconds

References

ab176819 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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