Overview

  • Product nameAnti-DHX29 antibody
    See all DHX29 primary antibodies
  • Description
    Rabbit polyclonal to DHX29
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to a region between residue 150 and 200 of Human DHX29 (NP_061903.2).

  • Positive control
    • HeLa and 293T cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 175 kDa (predicted molecular weight: 155 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceDHX29 belongs to the DEAD box helicase family in the DEAH subfamily. It contains 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DHX29 may be involved in ATP-dependent DNA or RNA unwinding.
  • Cellular localizationCytoplasmic (Probable)
  • Database links
  • Alternative names
    • DDX29 antibody
    • DEAH (Asp Glu Ala Asp/His) box polypeptide 29 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 29 antibody
    • DEAH box protein 29 antibody
    • Nucleic acid helicase DDXx antibody
    • Putative ATP dependent RNA helicase DHX29 antibody
    see all

Anti-DHX29 antibody images

  • All lanes : Anti-DHX29 antibody (ab70745) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 155 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg whole cell lysate fro HeLa cells was immunoprecipitated using ab70745 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70745 at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.

References for Anti-DHX29 antibody (ab70745)

ab70745 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS cells)
Total protein in input 500 µg
Immuno-precipitation step Protein A/G
Specification U2OS cells
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Submitted Jul 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS Cells)
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Loading amount 20 µg
Specification U2OS Cells
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"