The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500. Detects a band of approximately 175 kDa (predicted molecular weight: 155 kDa).
Use at 2-5 µg/mg of lysate.
DHX29 belongs to the DEAD box helicase family in the DEAH subfamily. It contains 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DHX29 may be involved in ATP-dependent DNA or RNA unwinding.
1mg whole cell lysate fro HeLa cells was immunoprecipitated using ab70745 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70745 at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.