Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX30
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 350-400 of Human DHX30 (NP_619520.1)

  • Positive control
    • Hela cell lysate, 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 5 - 10µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 134 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 134 kDa
      Observed band size : 134 kDa
      Additional bands at : 110 kDa,238 kDa,60 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.

    References

    This product has been referenced in:
    • Antonicka H  et al. A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. EMBO Rep 18:28-38 (2017). Read more (PubMed: 27974379) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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