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Rabbit polyclonal to DHX30
Predicted to work with:
Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human DHX30 (NP_619520.1)
Hela cell lysate, 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5 - 10µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 134 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Isoform 2 is phosphorylated on Ser-15.
Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid.
Information by UniProt
DEAH box protein 30 antibody
Western blot - DHX30 antibody (ab85687)
All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Predicted band size: 134 kDa Observed band size: 134 kDa Additional bands at: 110 kDa, 238 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - DHX30 antibody (ab85687)
Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.
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