All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 134 kDa Observed band size: 134 kDa Additional bands at: 110 kDa, 180 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation - DHX30 antibody (ab85754)
Detection of Human DHX3 by Western Blot of Immunprecipitate. All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies: Lane 1: ab85754 Lane 2: Control IgG Detection: Chemiluminescence with exposure time of 3 minutes.