Overview

  • Product name
    Anti-DHX32 antibody
  • Description
    Rabbit polyclonal to DHX32
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EEEGLECPNS SSEKRYFPES LDSSDGDEEE VLACEDLELN PFDGLPYSSR) of human DHX32 (NP_060650).

  • Positive control
    • Human fetal lung lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85459 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity
    Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3110079L04Rik antibody
    • AA408140 antibody
    • Ddx32 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32 antibody
    • DEAD/H box 32 antibody
    • DEAD/H helicase like protein 1 antibody
    • DEAD/H helicase-like protein 1 antibody
    • DEAH (Asp-Glu-Ala-His) box polypeptide 32 antibody
    • DEAH box protein 32 antibody
    • DHLP1 antibody
    • DHX32 antibody
    • DHX32_HUMAN antibody
    • Helicase DDX32 antibody
    • HuDDX32 antibody
    • Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 antibody
    see all

Images

  • ICC/IF image of ab85459 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85459 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-DHX32 antibody (ab85459) at 1 µg/ml + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Lin H  et al. DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ß-catenin Signaling to Induce Expression of VEGFA. EBioMedicine 18:62-72 (2017). Human . Read more (PubMed: 28330603) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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