Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX33
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between N terminal residues 1 and 50 of DHX33. (NP_064547.2)

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
    • Cellular localization
      Nucleus, nucleolus.
    • Database links
    • Alternative names
      • DDX33 antibody
      • DEAD box polypeptide 33 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 33 antibody
      • DEAH box protein 33 antibody
      • FLJ21972 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DHX33 with ab70863 at 1/1000 (1µg/ml). Detection: DAB.
    • Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: ab70863 (Lane 1) was used for IP at 3 µg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody (lane 2) and rabbit IgG control (Lane 3).
      Detection: Chemiluminescence with an exposure time of 3 minutes.

    References

    ab70863 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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