The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
Use a concentration of 2 - 5 µg/ml.
DHX33 is a DEAD box protein whose function has not yet been determined.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
DEAH (Asp Glu Ala His) box polypeptide 33 antibody
DEAH box protein 33 antibody
Western blot - DHX33 antibody (ab72451)
All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 79 kDa Observed band size : 79 kDa Additional bands at : 200 kDa,31 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - DHX33 antibody (ab72451)
Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
Lane 1: ab72451 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.