Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX33
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    A synthetic peptide corresponding to a region between residues 650 and the C terminus (residue 707) of human DHX33.

  • Positive control
    • Hela whole cell lysate; 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IP Use a concentration of 2 - 5 µg/ml.

Target

  • Relevance
    DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
  • Cellular localization
    Nucleus, nucleolus.
  • Database links
  • Alternative names
    • DDX33 antibody
    • DEAD box polypeptide 33 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 33 antibody
    • DEAH box protein 33 antibody
    • FLJ21972 antibody
    see all

Images

  • All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
    Additional bands at : 200 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
    Lane 1: ab72451 precipitate

    Lane 2: Control IgG

    For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab72451 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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