Anti-DHX36 antibody (ab226813)
Key features and details
- Rabbit polyclonal to DHX36
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DHX36 antibody
See all DHX36 primary antibodies -
Description
Rabbit polyclonal to DHX36 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human DHX36 (N terminal). The exact sequence is proprietary.
Database link: Q9H2U1 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 cell lysate. IHC: Mouse testis and rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 115 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 115 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 170506 Human
- Entrez Gene: 72162 Mouse
- Entrez Gene: 310461 Rat
- Omim: 612767 Human
- SwissProt: Q9H2U1 Human
- SwissProt: Q8VHK9 Mouse
- Unigene: 446270 Human
- Unigene: 224233 Mouse
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Alternative names
- DDX36 antibody
- DEAH box protein 36 antibody
- Dhx36 antibody
see all
Images
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All lanes : Anti-DHX36 antibody (ab226813) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa -
Paraffin-embedded mouse testis tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226813 has not yet been referenced specifically in any publications.