Overview

  • Product name
  • Description
    Rabbit polyclonal to DHX36
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region corresponding to C terminal amino acids 950-1008 of Human DHX36 (NP_065916.1)

  • Positive control
    • HeLa whole cell lysate NIH3T3 whole cell lysate 293T whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis.
  • Tissue specificity
    Highly expressed in testis.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDX36 antibody
    • DEAH box protein 36 antibody
    • Dhx36 antibody
    • DHX36_HUMAN antibody
    • EC 3.6.1.- antibody
    • KIAA1488 antibody
    • MLE like protein 1 antibody
    • MLE-like protein 1 antibody
    • MLEL1 antibody
    • Probable ATP dependent RNA helicase DHX36 antibody
    • Probable ATP-dependent RNA helicase DHX36 antibody
    • RHAU antibody
    • RNA helicase associated with AU rich element ARE antibody
    • RNA helicase associated with AU-rich element ARE antibody
    see all

Anti-DHX36 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DHX36 with ab70269 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DHX36 antibody (ab70269) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 115 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70269 at 3 µg/mg (lane 1) or control IgG (lane 2). 1/4 of the immunoprecipitate was loaded on to a gel, analysed by Western blot and revealed using ab70269 at 0.1 µg/ml.
  • ICC/IF image of ab70269 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70269, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70269 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70269, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DHX36 antibody (ab70269)

This product has been referenced in:
  • Kralovicova J & Vorechovsky I Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons. Nucleic Acids Res N/A:N/A (2016). Read more (PubMed: 27566151) »
  • Byrd AK  et al. Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress. J Biol Chem 291:18041-57 (2016). WB . Read more (PubMed: 27369081) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Ovary and testie)
Permeabilization
Yes - PBST
Specification
Ovary and testie
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 02 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Primary aleveolar epithelial cells)
Specification
Primary aleveolar epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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