Overview

  • Product name
    Anti-DHX37 antibody
  • Description
    Rabbit polyclonal to DHX37
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human DHX37 (NP_116045.2).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70778 was affinity purified using an epitope specific to DHX37 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      DHX37 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
    • Database links
    • Alternative names
      • DDX37 antibody
      • DEAH (Asp-Glu-Ala-His) box polypeptide 37 antibody
      • DEAH box protein 37 antibody
      • FLJ41974 antibody
      • Gm1050 antibody
      • Gm451 antibody
      • KIAA1517 antibody
      • MGC2695 antibody
      • MGC4322 antibody
      • MGC46245 antibody
      • MGC90028 antibody
      • mKIAA1517 antibody
      • Probable ATP dependent RNA helicase DHX37 antibody
      see all

    Images

    • All lanes : Anti-DHX37 antibody (ab70778) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 130 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of DHX37 by Western Blot of Immunprecipitate. ab70778 at 1µg/ml staining DHX37 in HeLa whole cell lysate immunoprecipitated using ab70778 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab70778 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70778.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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