Overview

  • Product nameAnti-DHX58 antibody
    See all DHX58 primary antibodies
  • Description
    Rabbit polyclonal to DHX58
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 12 amino acid synthetic peptide from near the carboxy terminus of human DHX58.

  • Positive control
    • Rat kidney tissue lysate. IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab67270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionParticipates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower.
  • Sequence similaritiesBelongs to the helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • D11LGP2 antibody
    • D11lgp2e antibody
    • DEXH (Asp Glu X His) box polypeptide 58 antibody
    • DEXH box polypeptide 58 antibody
    • DHX 58 antibody
    • DHX58 antibody
    • DHX58_HUMAN antibody
    • LGP 2 antibody
    • LGP2 antibody
    • Ortholog of mouse D11lgp2 antibody
    • Probable ATP dependent helicase LGP2 antibody
    • Probable ATP dependent RNA helicase DHX58 antibody
    • Probable ATP-dependent helicase LGP2 antibody
    • Probable ATP-dependent RNA helicase DHX58 antibody
    • Protein D11Lgp2 homolog antibody
    • RIG-I-like receptor LGP2 antibody
    • RLR antibody
    • RNA helicase LGP2 antibody
    see all

Anti-DHX58 antibody images

  • ICC/IF image of ab67270 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67270, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Anti-DHX58 antibody (ab67270) at 1 µg/ml
    Lane 2 : Anti-DHX58 antibody (ab67270) at 2 µg/ml

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Rat kidney tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit secondary antibody

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 80 kDa,90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DHX58 antibody (ab67270)

This product has been referenced in:
  • Childs KS  et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . Read more (PubMed: 23671710) »
  • Childs K  et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . Read more (PubMed: 22301134) »

See all 2 Publications for this product

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