Anti-DHX58/RLR antibody (ab67270)
Key features and details
- Rabbit polyclonal to DHX58/RLR
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-DHX58/RLR antibody
See all DHX58/RLR primary antibodies -
Description
Rabbit polyclonal to DHX58/RLR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
Target
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Function
Participates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower. -
Sequence similarities
Belongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 80861 Mouse
- Entrez Gene: 303538 Rat
- SwissProt: Q99J87 Mouse
- Unigene: 271830 Mouse
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Alternative names
- D11LGP2 antibody
- D11lgp2e antibody
- DEXH (Asp Glu X His) box polypeptide 58 antibody
see all
Images
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Lane 1 : Anti-DHX58/RLR antibody (ab67270) at 1 µg/ml
Lane 2 : Anti-DHX58/RLR antibody (ab67270) at 2 µg/ml
All lanes : Rat
kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab67270 has been referenced in 5 publications.
- Lenoir JJ et al. Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-κB activation. Cell Rep 37:110175 (2021). PubMed: 34965427
- Yamane D et al. Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses. Nat Microbiol 4:1096-1104 (2019). PubMed: 30988429
- Parisien JP et al. RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. EMBO Rep 19:N/A (2018). PubMed: 29661858
- Childs KS et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . PubMed: 23671710
- Childs K et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . PubMed: 22301134