Overview

  • Product nameAnti-DHX8 antibody
    See all DHX8 primary antibodies
  • Description
    Rabbit polyclonal to DHX8
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 325 and 375 of Human DHX8 (NP_004932.1)

  • Positive control
    • Human gastric tumor, Mouse squamous cell carcinoma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFacilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
    • Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 S1 motif domain.
    • DomainThe RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DHX8 antibody
      • ATP-dependent RNA helicase dhx8 antibody
      • DDX 8 antibody
      • DDX8 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase) antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 antibody
      • DEAD/H box 8 antibody
      • DEAD/H box polypeptide 8 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 8 antibody
      • DEAH box polypeptide 8 antibody
      • DEAH box protein 8 antibody
      • DHX 8 antibody
      • dhx8 antibody
      • DHX8_HUMAN antibody
      • HRH 1 antibody
      • HRH1 antibody
      • PRP 22 antibody
      • PRP22 antibody
      • PRP22, S. cerevisiae, homolog of antibody
      • PRPF 22 antibody
      • PRPF22 antibody
      • RNA helicase HRH 1 antibody
      • RNA helicase HRH1 antibody
      see all

    Anti-DHX8 antibody images

    • Detection of Human DHX8 by Immunohistochemistry on paraffin embedded formalin fixed section of Human gastric tumor using 1/500 ab84505. Detection: DAB staining
    • Detection of Mouse DHX8 by Immunohistochemistry on paraffin embedded formalin fixed section of Mouse squamous cell carcinoma using 1/100 ab84505. Detection: DAB staining

    References for Anti-DHX8 antibody (ab84505)

    ab84505 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Fruit fly (Drosophila melanogaster) Cell (Egg chamber)
    Specification Egg chamber
    Fixative Formaldehyde
    Permeabilization No
    Blocking step BSA + goat serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Mr. Stephen Klusza

    Verified customer

    Submitted Jul 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"