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Synthetic peptide, which represents a portion of the human diaphanous homolog 1 encoded within exon 27 (LocusLink ID 1729).
Our Abpromise guarantee covers the use of ab11173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||1/400. PubMed: 24302570|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/15000. Detects a band of approximately 200 kDa.|
The gel running conditions were reducing. The blocking time was 2 hours at 25°C.
The primary antibody was incubated for 16 hours at 4°C.