• Product nameAnti-DIAPH1 antibody
    See all DIAPH1 primary antibodies
  • Description
    Goat polyclonal to DIAPH1
  • Specificityab99499 is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 822-832 of Human DIAPH1 (NP_005210.3).

  • Positive control
    • NIH3T3 lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99499 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab99499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).


  • FunctionActs in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • Tissue specificityExpressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
  • Involvement in diseaseDefects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
  • Cellular localizationCell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DIAPH1 antibody
    • deafness, autosomal dominant 1 antibody
    • DFNA1 antibody
    • DIA1 antibody
    • DIAP1 antibody
    • DIAP1_HUMAN antibody
    • DIAPH1 antibody
    • Diaphanous homolog 1 (Drosophila) antibody
    • diaphanous homolog 1 antibody
    • Diaphanous related formin 1 antibody
    • Diaphanous-related formin-1 antibody
    • DRF1 antibody
    • FLJ25265 antibody
    • hDIA1 antibody
    • LFHL1 antibody
    • low frequency hearing loss 1 antibody
    • p140DIA antibody
    • Protein diaphanous homolog 1 antibody
    see all

Anti-DIAPH1 antibody images

  • Anti-DIAPH1 antibody (ab99499) at 0.3 µg/ml (for 1 hour) + NIH3T3 lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

References for Anti-DIAPH1 antibody (ab99499)

ab99499 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99499.
Please use the links above to contact us or submit feedback about this product.