Overview

  • Product nameAnti-DIAPH2 antibody
    See all DIAPH2 primary antibodies
  • Description
    Rabbit polyclonal to DIAPH2
  • SpecificityThis antibody detects a band at 130kD in HEK293 cells representing DIA2.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    VPLERSRSRHNGAISSK

    conjugated to KLH, corresponding to amino acids 1085-1101 of Human DIA.

  • Positive control
    • HEK293 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab10562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/6000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

Target

  • FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Anti-DIAPH2 antibody images



  • Predicted band size : 132 kDa


    Western blot using ab10562 on HEK293 whole cell lysate at 1/500. Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 1 minute Expected molecular weight: 132 kDa 20µg of cell lysate per lane

References for Anti-DIAPH2 antibody (ab10562)

ab10562 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your recent e-mail. I have looked into your concerns very carefully and agree with you that the terminology can be very confusing. Following a BLAST search with the immunogen I can confirm the following: The immunogen sequence used t...

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