Overview

  • Product nameAnti-DIAPH2 antibody
    See all DIAPH2 primary antibodies
  • Description
    Rabbit polyclonal to DIAPH2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to C terminal amino acids of Mouse DIAPH2.

  • Positive control
    • A431, HeLa, Mouse brain tissue, C2C12 cells, Rat PC12 and Rabbit spleen fibroblast lysates.
  • General notesDo not aliquot. Stable for 1 year.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 126 kDa).

Target

  • FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Anti-DIAPH2 antibody images

  • All lanes : Anti-DIAPH2 antibody (ab119702) at 1/500 dilution

    Lane 1 : Rat PC12 lysate
    Lane 2 : Human A431 lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rabbit spleen fibroblast lysate


    Predicted band size : 126 kDa

References for Anti-DIAPH2 antibody (ab119702)

ab119702 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Ovary)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Ovary
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Apr 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"