Overview

  • Product name
  • Description
    Rabbit polyclonal to DIAPH2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to C terminal amino acids of Mouse DIAPH2.

  • Positive control
    • A431, HeLa, Mouse brain tissue, C2C12 cells, Rat PC12 and Rabbit spleen fibroblast lysates.
  • General notes
    Do not aliquot. Stable for 1 year.

Properties

Applications

Our Abpromise guarantee covers the use of ab119702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 126 kDa).

Target

  • Function
    Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificity
    Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in disease
    Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stage
    Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • Domain
    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localization
    Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Images

  • All lanes : Anti-DIAPH2 antibody (ab119702) at 1/500 dilution

    Lane 1 : Rat PC12 lysate
    Lane 2 : Human A431 lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rabbit spleen fibroblast lysate


    Predicted band size : 126 kDa

References

ab119702 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Ovary
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

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Submitted Apr 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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