Anti-DIAPH2 antibody (ab12319)

Overview

  • Product nameAnti-DIAPH2 antibody
    See all DIAPH2 primary antibodies
  • Description
    Rabbit polyclonal to DIAPH2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human diaphanous homolog 2 encoded within exon 3 (LocusLink ID 1730).

  • Positive control
    • HEK 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab12319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 132 kDa.

Target

  • FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Anti-DIAPH2 antibody images



  • Predicted band size : 132 kDa


    Detection of Human Diaphanous 2 by Western Blot.
    Samples: Whole cell lysate (50 mcg) from HEK 293T cells.
    Antibody: ab12319 used at the indicated concentrations.
    Detection: Chemiluminescence.
  • IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DIAPH2 antibody (ab12319)

ab12319 has not yet been referenced specifically in any publications.

Product Wall

We have added the blocking peptides for the two DIAPH2 antibodies you asked about to our catalogue.

DIAPH2 peptide (ab156989) for ab12319
DIAPH2 peptide (ab156990) for ab102841

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Vein Endothelial Cells)
Loading amount 50 µg
Specification Human Vein Endothelial Cells
Gel Running Conditions Reduced Denaturing (7.5 %)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Elena Astanina

Verified customer

Submitted Feb 23 2012

Thank you for your patience in this matter. I have received a reply from the originator of ab12319 (DIAPH2 antibody). The immunogenic peptide sequence is proprietary information. However, they indicated that this antibody recognizes an epitope tha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"