Anti-DIAPH3 antibody (ab77678)

Overview

  • Product nameAnti-DIAPH3 antibody
    See all DIAPH3 primary antibodies
  • Description
    Mouse monoclonal to DIAPH3
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: PDILNFVDDL EPLDKASKVS VETLEKNLRQ MGRQLQQLEK ELETFPPPED LHDKFVTKMS RFVISAKEQY ETLSKLHENM EKLYQSIIGY YAIDVKKV, corresponding to amino acids 632-729 of Human DIAPH3 (NP_112194), with a 26kDa enzyme tag

Properties

Applications

Our Abpromise guarantee covers the use of ab77678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
    • Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
      Contains 1 DAD (diaphanous autoregulatory) domain.
      Contains 1 FH1 (formin homology 1) domain.
      Contains 1 FH2 (formin homology 2) domain.
      Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
    • DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
    • Information by UniProt
    • Database links
    • Alternative names
      • AN antibody
      • AUNA1 antibody
      • Dia2 antibody
      • diap3 antibody
      • DIAP3_HUMAN antibody
      • DIAPH3 antibody
      • Diaphanous homolog 3 (Drosophila) antibody
      • Diaphanous homolog 3 antibody
      • Diaphanous related formin 3 antibody
      • Diaphanous, Drosophila, homolog of, 3 antibody
      • Diaphanous-related formin-3 antibody
      • DKFZp434C0931 antibody
      • DKFZp686A13178 antibody
      • DRF3 antibody
      • FLJ34705 antibody
      • mDia2 antibody
      • NSDAN antibody
      • OTTHUMP00000018480 antibody
      • Protein diaphanous homolog 3 antibody
      • RP11-26P21.1 antibody
      see all

    Anti-DIAPH3 antibody images

    • Anti-DIAPH3 antibody (ab77678) at 1/500 dilution + Recombinant fragment of human DIAPH3 at 0.2 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

    References for Anti-DIAPH3 antibody (ab77678)

    ab77678 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (SW620)
    Specification SW620
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Mouse Cell lysate - whole cell (wt MEFs)
    Specification wt MEFs
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2013

    Application Western blot
    Sample Human Cell lysate - whole cell (MD-MB231 HeLa)
    Loading amount 30 µg
    Specification MD-MB231 HeLa
    Gel Running Conditions Reduced Denaturing (4 - 20 % gel)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Pilans Didzis

    Verified customer

    Submitted May 15 2013

    Since the immunogen for ab77678 is from aa 632 - 729, the antibody is capable of detecting all 7 isofroms of DIAPH3 (98, 79, 137, 135, 131, 129, 128 kDa for isoforms 1-7, respectively according to UniProt, link below)
    http://www.uniprot.org/uniprot...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"