Anti-Diazepam Binding Inhibitor antibody [20B10] (ab16775)


  • Product nameAnti-Diazepam Binding Inhibitor antibody [20B10]
    See all Diazepam Binding Inhibitor primary antibodies
  • Description
    Mouse monoclonal [20B10] to Diazepam Binding Inhibitor
  • Tested applicationsSuitable for: Flow Cyt, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Fusion protein (Human DBI).

  • Positive control
    • HepG2 whole cell lysate.



Our Abpromise guarantee covers the use of ab16775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 1 - 2 µg/ml.


  • FunctionBinds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
  • Tissue specificityIsoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.
  • Sequence similaritiesBelongs to the ACBP family.
    Contains 1 ACB (acyl-CoA-binding) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACBD 1 antibody
    • ACBD1 antibody
    • ACBP antibody
    • ACBP_HUMAN antibody
    • Acyl CoA binding domain containing 1 antibody
    • Acyl CoA binding protein antibody
    • Acyl Coenzyme A binding domain containing 1 antibody
    • Acyl coenzyme A binding protein antibody
    • Acyl-CoA-binding protein antibody
    • CCK RP antibody
    • CCKRP antibody
    • Cholecystokinin releasing peptide trypsin sensitive antibody
    • DBI antibody
    • Diazepam-binding inhibitor antibody
    • Endozepine antibody
    • EP antibody
    • GABA receptor modulator antibody
    • MGC70414 antibody
    see all

Anti-Diazepam Binding Inhibitor antibody [20B10] images

  • Overlay histogram showing HepG2 cells stained with ab16775 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16675, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunoprecipitation analysis of HepG2 cell lysates using ab16775.

    Lane 1: Input.

    Lane 2: Precipitates.

References for Anti-Diazepam Binding Inhibitor antibody [20B10] (ab16775)

ab16775 has not yet been referenced specifically in any publications.

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