Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)

Abreviews (1) Submit a Question Specific References (1)

Overview

  • Product name
    Anti-Diazepam Binding Inhibitor antibody [27C9]
    See all Diazepam Binding Inhibitor primary antibodies
  • Description
    Mouse monoclonal [27C9] to Diazepam Binding Inhibitor
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Fusion protein (Human DBI) purified from E.coli.

  • Positive control
    • HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IP
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml lysate.
    WB: 1/100 - 1/500. Predicted molecular weight: 10 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
    • Tissue specificity
      Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.
    • Sequence similarities
      Belongs to the ACBP family.
      Contains 1 ACB (acyl-CoA-binding) domain.
    • Information by UniProt
    • Database links
      • Alternative names
        • ACBD 1 antibody
        • ACBD1 antibody
        • ACBP antibody
        • ACBP_HUMAN antibody
        • Acyl CoA binding domain containing 1 antibody
        • Acyl CoA binding protein antibody
        • Acyl Coenzyme A binding domain containing 1 antibody
        • Acyl coenzyme A binding protein antibody
        • Acyl-CoA-binding protein antibody
        • CCK RP antibody
        • CCKRP antibody
        • Cholecystokinin releasing peptide trypsin sensitive antibody
        • DBI antibody
        • Diazepam-binding inhibitor antibody
        • Endozepine antibody
        • EP antibody
        • GABA receptor modulator antibody
        • MGC70414 antibody
        see all

      Images

      • Western blot - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        Western blot - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806) + HepG2

        Predicted band size: 10 kDa



        Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
        IHC-P image of Diazepam Binding Inhibitor antibody (ab16806) on Lung cancer sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.

        See Abreview

      • Immunoprecipitation - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        Immunoprecipitation - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)

        Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



        All lanes : Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806) at 1/250 dilution

        Lane 1 : HepG2 Cell lysate
        Lanes 2-3 : Precipitated from HepG2 Cell lysate at 200 µg
        Lane 4 : Precipitated from PBS
      • Western blot - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        Western blot - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
        ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL). ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL).

      References

      This product has been referenced in:
      • Harris FT  et al. Acyl-coenzyme A-binding protein regulates Beta-oxidation required for growth and survival of non-small cell lung cancer. Cancer Prev Res (Phila) 7:748-57 (2014). Read more (PubMed: 24819876) »

      See 1 Publication for this product

      Publishing research using ab16806? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

      10
      Votes: Highest First

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Diazepam Binding Inhibitor antibody [27C9]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Lung malignant)
      Specification
      Lung malignant
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
      Permeabilization
      No
      Blocking step
      H2O2 as blocking agent for 10 mins · Concentration: 3 % · Temperature: 22°C as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
      Read More
      abreview image
      Username

      Dr. Aamir Ahmed

      Verified customer

      Submitted Jan 25 2011

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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