Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)

Overview

  • Product name
    Anti-Diazepam Binding Inhibitor antibody [27C9]
    See all Diazepam Binding Inhibitor primary antibodies
  • Description
    Mouse monoclonal [27C9] to Diazepam Binding Inhibitor
  • Tested applications
    Suitable for: ELISA, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Fusion protein (Human DBI) purified from E.coli.

  • Positive control
    • HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IP
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml lysate.
    WB: 1/100 - 1/500. Predicted molecular weight: 10 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
    • Tissue specificity
      Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.
    • Sequence similarities
      Belongs to the ACBP family.
      Contains 1 ACB (acyl-CoA-binding) domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACBD 1 antibody
      • ACBD1 antibody
      • ACBP antibody
      • ACBP_HUMAN antibody
      • Acyl CoA binding domain containing 1 antibody
      • Acyl CoA binding protein antibody
      • Acyl Coenzyme A binding domain containing 1 antibody
      • Acyl coenzyme A binding protein antibody
      • Acyl-CoA-binding protein antibody
      • CCK RP antibody
      • CCKRP antibody
      • Cholecystokinin releasing peptide trypsin sensitive antibody
      • DBI antibody
      • Diazepam-binding inhibitor antibody
      • Endozepine antibody
      • EP antibody
      • GABA receptor modulator antibody
      • MGC70414 antibody
      see all

    Images

    • Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806) at 250 + HepG2

      Predicted band size : 10 kDa

      Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

    • IHC-P image of Diazepam Binding Inhibitor antibody (ab16806) on Lung cancer sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.

      See Abreview

    • Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175



    • Predicted band size : 10 kDa
      ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL). ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL).

    References

    This product has been referenced in:
    • Harris FT  et al. Acyl-coenzyme A-binding protein regulates Beta-oxidation required for growth and survival of non-small cell lung cancer. Cancer Prev Res (Phila) 7:748-57 (2014). Read more (PubMed: 24819876) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung malignant)
    Specification
    Lung malignant
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
    Permeabilization
    No
    Blocking step
    H2O2 as blocking agent for 10 mins · Concentration: 3 % · Temperature: 22°C as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Dr. Aamir Ahmed

    Verified customer

    Submitted Jan 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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