Overview

  • Product nameAnti-Dicer antibody
    See all Dicer primary antibodies
  • Description
    Rabbit polyclonal to Dicer
  • SpecificityThere are multiple bands in the blot shown, though there is a band at the appropriate predicted size (255 kDa).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from residues 900 - 1000 Dicer (Dcr1).

    .

  • Positive control
    • This antibody gave a positive signal in drosophila embryo lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab4735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 255 kDa (predicted molecular weight: 255 kDa).

Target

  • FunctionDouble-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Involvement in diseasePleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • Sequence similaritiesBelongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • DCR antibody
      • DCR1 antibody
      • Dicer 1 ribonuclease type III antibody
      • Dicer antibody
      • DICER_HUMAN antibody
      • DICER1 antibody
      • Double-strand-specific ribonuclease antibody
      • Endoribonuclease Dicer antibody
      • Helicase MOI antibody
      • Helicase with RNase motif antibody
      • HERNA antibody
      • KIAA0928 antibody
      • MNG1 antibody
      • RMSE2 antibody
      see all

    Anti-Dicer antibody images

    • Lanes 1 - 2 : Anti-Dicer antibody (ab4735) at 1/500 dilution
      Lanes 3 - 4 : Anti-Dicer antibody (ab4735) at 1/1000 dilution

      Lane 1 : 5 ug of drosophila embryo lysate.
      Lane 2 : 5 ug of drosophila embryo lysate.
      Lane 3 : 5 ug of drosophila embryo lysate.
      Lane 4 : 5 ug of drosophila embryo lysate.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 255 kDa
      Observed band size : 255 kDa
      Additional bands at : 170 kDa (possible cleavage fragment,cross reactivity).

      Exposure time : 30 seconds

    References for Anti-Dicer antibody (ab4735)

    This product has been referenced in:
    • Miles WO  et al. Pumilio facilitates miRNA regulation of the E2F3 oncogene. Genes Dev 26:356-68 (2012). WB ; Drosophila melanogaster . Read more (PubMed: 22345517) »
    • Kibanov MV  et al. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Mol Biol Cell 22:3410-9 (2011). Drosophila melanogaster . Read more (PubMed: 21775629) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry. At Abcam we always interested in improving the quality of information on our datasheets. To this effect I would be very interested in determining the cross species reactivity of ab4735. Please can you tell me the application...

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