Anti-Differentiated Endothelial Cells antibody [1F10] (ab15634)

Overview

  • Product nameAnti-Differentiated Endothelial Cells antibody [1F10]
  • Description
    Rat monoclonal [1F10] to Differentiated Endothelial Cells
  • Specificityab15634 recognises an antigen on human continuous blood endothelial cells, which are fully differentiated and does not stain any other cell in tissues or in peripheral blood.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Cultured HUVECs.

  • Positive control
    • Continuous endothelium of Spleen, Liver, Kidney. Stromal cells of stomach and mammary carcinoma.
  • General notesThe antigen is an SDS sensitive 250kD surface protein as determined by gel filtration. The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigen expression on cultured HUVEC is dependent on the culture conditions. Best results are obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercial available specific endothelial cell media have no influence on the antigen expression.

Properties

Applications

Our Abpromise guarantee covers the use of ab15634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
  • Application notesIHC-Fr: Use at a concentration of 2 µg/ml (1:100). Fixation: Acetone: 5 min at 4°C.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe endothelium is the layer of thin, flat cells that lines the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall. Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. In small blood vessels and capillaries, endothelial cells are often the only cell-type present. Endothelial cells are involved in many aspects of vascular biology, including: vasoconstriction & vasodilation, blood clotting (thrombosis & fibrinolysis), atherosclerosis, formation of new blood vessels (angiogenesis) and inflammation and swelling (edema). Endothelial cells also control the passage of materials — and the transit of white blood cells — into and out of the bloodstream. In some organs, there are highly differentiated endothelial cells to perform specialized 'filtering' functions. Examples of such unique endothelial structures include the renal glomerulus and the blood-brain barrier.

    References for Anti-Differentiated Endothelial Cells antibody [1F10] (ab15634)

    ab15634 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry. My colleagues from the lab where the antibody [1F10] ab15634 is produced said that this antibody shows a faint staining with lymphatic endothelial cells of the skin. Other reactions with lymphatic endothelial cells ar...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"