Overview

  • Product nameAnti-Digoxigenin antibody [21H8]
    See all Digoxigenin primary antibodies
  • Description
    Mouse monoclonal [21H8] to Digoxigenin
  • SpecificityThis antibody reacts with free and bound digoxigenin.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, ICC/IF, Southern Blotmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number21H8
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
In situ hybridization Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23508956
Southern Blot 1/500 - 1/5000.

Target

  • RelevanceDigoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

References for Anti-Digoxigenin antibody [21H8] (ab420)

This product has been referenced in:
  • Gallo A  et al. Targeting the Ca(2+) Sensor STIM1 by Exosomal Transfer of Ebv-miR-BART13-3p is Associated with Sjögren's Syndrome. EBioMedicine 10:216-26 (2016). Read more (PubMed: 27381477) »
  • Zhou X  et al. MicroRNA-7 Inhibits Tumor Metastasis and Reverses Epithelial-Mesenchymal Transition through AKT/ERK1/2 Inactivation by Targeting EGFR in Epithelial Ovarian Cancer. PLoS One 9:e96718 (2014). IHC . Read more (PubMed: 24816687) »

See all 10 Publications for this product

Product Wall

Thank you very much for your call yesterday and for your patience while I've been in touch with the lab about this antibody.

The antibody is protein A purified then lyophilized from a buffer ofTris 0.1M, glycine 0.1M, sucrose 2%,andit is late...

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Thank you for contacting us.
Technically the charge on antibodies at pH7 should be neutral however it depends on antibody.
We do not have any information about charge for ab420 so I am sorry we are unable to share any information.
I hope ...

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Thank you for contacting Abcam regarding ab420.


The antibody has previously been sold as an ascitic fluid (concentration not determined because not relevant).


More recently we have provided protein A purified antibody packag...

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Vielen Dank für Ihre Antwort.

Ich kann Ihnen ein Röhrchen von einem der folgenden Antiköpern anbieten. Alle sind gegen Maus IgG1 gerichtet, FITC konjugiert und für Flow Cytometry getestet und garantiert:

ab97239 ...

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Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Ihr versuch nicht optimal läuft.

Wenn ich Sie richtig verstehen haben sie den sekundären Antikörper (ab99900) mit einem anderen primären Antikörper ...

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Vielen Dank für Ihre Antwort.

Es freut mich zu hören, dass das Experiment mit einem unkonjugierten Antikörperfunktioniert hat.

Leider ist es nicht möglich die Konzentration von ab420 zu bestimmen. Bei ab420 hande...

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Thank you for contacting us. For a valid comparison, we would need to test the two antibodies together in the same assay on the same material. Sometimes a dissociation constant is calculated for antibodies and the antigens they bind to, which a...

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Thank you for your enquiry. I have been in contact with the originator of ab420 Digoxigenin antibody [21H8] and I can confirm that this has been tested for this application (anti-dig-dig labeled ssDNA interaction) with a hybridisation buffer SSC 5X...

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Thank you for your enquiry. I can confirm that this product contains the Fc fragment.

Thank you for your enquiry. The hybridoma used to make this product was developed from mice as indicated on the data sheet. The immunogen (digoxigenin) was used following the standard protocol for the development of monoclonal antibody. Then fusion ...

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1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"