After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the Digoxigenin immobilized on a solid support.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues.
DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.