Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 - 2 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
    • Alternative names
      • 1672-46-4 antibody
      • BRN 0096479 antibody
      • DIG antibody
      • EINECS 216-806-2 antibody
      • HSDB 7108 antibody
      • Lanadigenin antibody
      • Lanadigigenin antibody
      • ST056392 antibody
      see all

    References

    This product has been referenced in:
    • Huang J  et al. The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand crosslinks. Mol Cell 52:434-46 (2013). Read more (PubMed: 24207054) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Formaldehyde
    Username

    Mr. Joseph Polex Wolf

    Verified customer

    Submitted Aug 15 2014

    Thank you for contacting us. I am sorry to hear that you have encountered problems wit this antibody. According to the datasheet (http://www.abcam.com/digoxigenin-antibody-ab51946.html), the recommended storage temperature is 4 degrees, not -20 degre...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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