Anti-Digoxigenin antibody (ab76907)
Key features and details
- Goat polyclonal to Digoxigenin
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Digoxigenin antibody
See all Digoxigenin primary antibodies -
Description
Goat polyclonal to Digoxigenin -
Host species
Goat -
Specificity
ab76907 reacts 100% with digoxigenin and digoxin, it does not cross-react with the carrier protein KLH or other steroids. -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Digoxigenin conjugated to keyhole limpet haemocyanin. Digoxigenin conjugated to KLH.
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Positive control
- Here are some suggestions: In IHC digoxigenin-labeled proteins: 1 µg/ml - 2 µg/ml. In WB digoxigenin-labeled proteins: 0.5 µg/ml – 2 µg/ml. In Situ hybridization: Digoxigenin-labeled nucleic acid 0.1 µg/ml - 0.8 µg/ml. The optimum dilution should be determined by the individual lab.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
ab76907 is purified from immunized serum by ion exchange chromatography and immunoaffinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/40000. Detects a band of approximately 150 kDa.
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ELISA |
1/100000 - 1/1e+006.
Digoxigenin-BSA at 2-5 µg/ml, apply 50 µl/well in ELISA. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/40000. Detects a band of approximately 150 kDa. |
ELISA
1/100000 - 1/1e+006. Digoxigenin-BSA at 2-5 µg/ml, apply 50 µl/well in ELISA. |
Target
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Relevance
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab76907 has been referenced in 14 publications.
- Hiebert P et al. Activation of Nrf2 in fibroblasts promotes a skin aging phenotype via an Nrf2-miRNA-collagen axis. Matrix Biol 113:39-60 (2022). PubMed: 36367485
- González-Acosta D et al. PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. EMBO J 40:e106355 (2021). PubMed: 34128550
- Graves D et al. Differential Splicing of Human Adenovirus 5 E1A RNA Expressed in cis versus in trans. J Virol 95:N/A (2021). PubMed: 33361423
- Shibata T et al. Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model. Nat Commun 12:236 (2021). PubMed: 33431896
- Ma G et al. Simultaneous Imaging of Single Protein Size, Charge, and Binding Using A Protein Oscillation Approach. Bio Protoc 11:e3934 (2021). PubMed: 33796608