Overview

  • Product name
  • Description
    Goat polyclonal to Digoxigenin
  • Specificity
    ab76907 reacts 100% with digoxigenin and digoxin, it does not cross-react with the carrier protein KLH or other steroids.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with Digoxigenin
  • Immunogen

    Digoxigenin conjugated to KLH.

  • Positive control
    • Digoxigenin-BSA at 2-5 µg/ml, apply 50µl/well in ELISA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    ab76907 is purified from immunized serum by ion exchange chromatography and immunoaffinity chromatography
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/40000. Detects a band of approximately 150 kDa.
ELISA 1/100000 - 1/1e+006.

Target

  • Relevance
    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

References for Anti-Digoxigenin antibody (ab76907)

This product has been referenced in:
  • Moers AP  et al. Detection of single-nucleotide polymorphisms in Plasmodium falciparum by PCR primer extension and lateral flow immunoassay. Antimicrob Agents Chemother 59:365-71 (2015). Read more (PubMed: 25367901) »
  • Hu F  et al. Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization. Oncol Lett 9:2795-2798 (2015). IHC-P . Read more (PubMed: 26137149) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Joseph Polex Wolf

Verified customer

Submitted Aug 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Blocking step
Blocking reagent (Roche) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2012

Thank you for your enquiry. It has taken some time to obtain some information for you, and I apologise for the delay in responding and appreciate your patience.

I am sorry to confirm that as far as we are aware, none of our digoxigenin antib...

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I have published your Abreview by changing application from IHC-Fr to IHC-P as per your request; you will be able to get the free antibody now.

Thanks!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver, lung)
Specification
liver, lung
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Boehringer Blocking reagent as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2012

This is fine! please submit abreview for IHC-P application.

The free antibody code will be valid only, when your abreview gets published. So please submit your Abreview we will publish it asap.

Thank you for your email.

Please submit the abreview under the more closer IHC-Fr application.

Please do not forget to provide summary of the application you have used in additional notes box. Once we receive the abreview we will then...

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DISCOUNT CODE: XXXXXXXXXX
Expiration date: 22-08-2012

I am very pleased to hear you would like to use ab76907 in ISH (In situ hybridization)and send us an image of your results.

This code will give you 1 free [PRIMARY ANTIBOD...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for contacting us.

Indeed HRP conjugated secondary antibody can be used for target localization after insitu hybridization. Please check the PubMed literature there are many publications available where scientist have used HRP conj...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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