Anti-Digoxigenin antibody [BT.21H8] (Biotin) (ab419)


  • Product nameAnti-Digoxigenin antibody [BT.21H8] (Biotin)
    See all Digoxigenin primary antibodies
  • Description
    Mouse monoclonal [BT.21H8] to Digoxigenin (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Immunogen

    Synthetic peptide corresponding to Digoxigenin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purification notesPurified biotin conjugate.
  • ClonalityMonoclonal
  • Clone numberBT.21H8
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21813679


  • RelevanceDigoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

References for Anti-Digoxigenin antibody [BT.21H8] (Biotin) (ab419)

This product has been referenced in:
  • Heine S  et al. CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity. J Neurosci 31:11184-92 (2011). IHC-FoFr . Read more (PubMed: 21813679) »
  • Silva VA  et al. Expression and cellular localization of microRNA-29b and RAX, an activator of the RNA-dependent protein kinase (PKR), in the retina of streptozotocin-induced diabetic rats. Mol Vis 17:2228-40 (2011). In situ hybridization . Read more (PubMed: 21897745) »

See all 3 Publications for this product

Product Wall

Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Ihr versuch nicht optimal läuft.

Wenn ich Sie richtig verstehen haben sie den sekundären Antikörper (ab99900) mit einem anderen primären Antikörper ...

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Vielen Dank für Ihre Antwort.

Es freut mich zu hören, dass das Experiment mit einem unkonjugierten Antikörperfunktioniert hat.

Leider ist es nicht möglich die Konzentration von ab420 zu bestimmen. Bei ab420 hande...

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Vielen Dank für Ihre Anfrage.

Wenn Antikörper mit Biotin gekoppelt werden, wird das meist über freie Anime getan. Das bedeutet das Biotin über den ganzen Antikörper verteilt ist. Die Antikörper werden dann darauf...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (thymus, eye)
Specification thymus, eye
Fixative Paraformaldehyde
Permeabilization Yes - tritonX-100 10min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Lampros Panagis

Verified customer

Submitted Feb 10 2011

Thank you for your enquiry. We do not provide MS data sheets for our products. All necessary information is located on the product datasheet (which can be viewed online or was shipped with the product).

We are sorry to inform you that this data is not available.

My apologies ab419 quite obivously is biotinylated. My only excuse is that for some reason our internal system isn't showing the conjugate when I search. The antibody is guaranteed for what it says on the datasheet i.e tested applications, species etc ...

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