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Our Abpromise guarantee covers the use of ab6212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ELISA||1/1000 - 1/10000.|
|IHC-P||1/200 - 1/2000. Use in combination with tyramide signal amplification.|
|IHC-Fr||1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22393045|
|In situ hybridization||Use at an assay dependent dilution. PubMed: 20019669|
ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.
This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.