Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)

Overview

  • Product name
    Anti-Dihydrofolate reductase (DHFR) antibody
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Rabbit polyclonal to Dihydrofolate reductase (DHFR)
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-187 of Human Dihydrofolate reductase (DHFR) (Uniprot ID: P00374).

  • Positive control
    • Human colon carcinoma tissue; A431 cells; 293T, A431, Jurkat, Raji whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab152159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/200 dilution.
  • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab152159) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa

References

ab152159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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