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Rabbit polyclonal to Dihydrofolate reductase (DHFR)
HumanPredicted to work with:
Mouse, Rat, Cow
Recombinant fragment corresponding to a region within amino acids 1-187 of Human Dihydrofolate reductase (DHFR) (Uniprot ID: P00374).
- Human colon carcinoma tissue; A431 cells; 293T, A431, Jurkat, Raji whole cell lysates.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 21 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
||1/100 - 1/1000.
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
Information by UniProt
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/200 dilution.
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab152159) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"