Western blot - Anti-Dihydrofolate reductase (DHFR) antibody (ab49881)
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml
Lane 1 : recombinant DHFR at 0.2 µg Lane 2 : recombinant DHFR at 0.1 µg Lane 3 : recombinant DHFR at 0.05 µg Lane 4 : recombinant DHFR at 0.025 µg Lane 5 : recombinant DHFR at 0.0125 µg
Developed using the ECL technique.
Predicted band size: 21 kDa Observed band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody (ab49881)This image is courtesy of an anonymous abreview.
ab49881 at a 1/30 dilution staining Dihydrofolate reductase (DHFR) in human squamous cell carcinoma tissue by Immunohistochemistry/ Formalin fixed, paraffin embedded, incubated for 12 hours at 4°C. Heat mediated antigen retrieval step performed using EDTA pH 9.0. Blocked with 1% milk for 30 minutes at 25°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to biotin.
Lee YH et al. DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells. Oncol Lett14:171-179 (2017).
Read more (PubMed: 28693150) »
Youn JY et al. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol29:1123-33 (2015).
Read more (PubMed: 26121233) »