Overview

  • Product nameAnti-Dihydrofolate reductase (DHFR) antibody
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Mouse monoclonal to Dihydrofolate reductase (DHFR)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: HFLSRSLDDA LKLTEQPELA NKVDMVWIVG GSSVYKEAMN HPGHLKLFVT RIMQDFESDT FFPEIDLEKY KLLPEYPGVL SDVQEEKGIK YKFEVYEKND , corresponding to amino acids 88-188 of Human Dihydrofolate reductase (DHFR)

Properties

Applications

Our Abpromise guarantee covers the use of ab54612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
  • Sequence similaritiesBelongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHFR antibody
    • DHFRP1 antibody
    • Dihydrofolate reductase antibody
    • DYR antibody
    • DYR_HUMAN antibody
    • EC 1.5.1.3 antibody
    see all

Anti-Dihydrofolate reductase (DHFR) antibody images



  • Predicted band size : 21 kDa
  • ab54612 at 10 µg/ml staining Dihydrofolate reductase (DHFR) in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-Dihydrofolate reductase (DHFR) antibody (ab54612)

This product has been referenced in:
  • Serova M  et al. Single agent and combination studies of pralatrexate and molecular correlates of sensitivity. Br J Cancer 104:272-80 (2011). WB ; Human . Read more (PubMed: 21179031) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"