Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)

Overview

  • Product nameAnti-Dihydrofolate reductase (DHFR) antibody
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Rabbit polyclonal to Dihydrofolate reductase (DHFR)
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Dihydrofolate reductase (DHFR) (internal sequence).

  • Positive control
    • Breast Carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab82171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
  • Sequence similaritiesBelongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHFR antibody
    • DHFRP1 antibody
    • Dihydrofolate reductase antibody
    • DYR antibody
    • DYR_HUMAN antibody
    • EC 1.5.1.3 antibody
    see all

Anti-Dihydrofolate reductase (DHFR) antibody images

  • Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with ab82171 at 1/50 dilution. Incubate primary antibody (ab82171) for 30 minutes at RT.
  • ICC/IF image of ab82171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82171, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)

This product has been referenced in:
  • Uramoto H  et al. TS, DHFR and GARFT expression in non-squamous cell carcinoma of NSCLC and malignant pleural mesothelioma patients treated with pemetrexed. Anticancer Res 30:4309-15 (2010). IHC ; Human . Read more (PubMed: 21036757) »

See 1 Publication for this product

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Thank you for your enquiry.

I have reviewed the immunogen sequences of the DHFR antibodies we have and regrettably the alignment with the e coli amino acid sequence is very low. Therefore, we would not anticipate that these antibodies would ...

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