Anti-Dihydrofolate reductase (DHFR) antibody (ab85056)

Overview

  • Product nameAnti-Dihydrofolate reductase (DHFR) antibody
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Rabbit polyclonal to Dihydrofolate reductase (DHFR)
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Dihydrofolate reductase (DHFR).

    (Peptide available as ab94547.)

  • Positive control
    • This antibody gave a positive signal in Human liver tissue lysate, and in the following whole cell lysates: HepG2; HeLa; Jurkat; MCF7.

Properties

Applications

Our Abpromise guarantee covers the use of ab85056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

Anti-Dihydrofolate reductase (DHFR) antibody images

  • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab85056) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 36 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab85056 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85056, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HepG2, and HeLa cells at 5µg/ml.

References for Anti-Dihydrofolate reductase (DHFR) antibody (ab85056)

ab85056 has not yet been referenced specifically in any publications.

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