Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)

Overview

  • Product nameAnti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR)
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Dihydrofolate reductase (DHFR).

  • Positive control
    • HeLa, Jurkat, Y-79, 293T, Mouse spleen and Rat kidney lysates; Human cervical carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/25 - 1/50.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] images

    • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : Y-79 cell lysates
      Lane 4 : 293T cell lysates
      Lane 5 : Mouse spleen lysates
      Lane 6 : Rat kidney lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
    • ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

    References for Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)

    This product has been referenced in:
    • Allen MD  et al. Prognostic and therapeutic impact of argininosuccinate synthetase 1 control in bladder cancer as monitored longitudinally by PET imaging. Cancer Res 74:896-907 (2014). Read more (PubMed: 24285724) »
    • Garon EB  et al. The HSP90 inhibitor NVP-AUY922 potently inhibits non-small cell lung cancer growth. Mol Cancer Ther 12:890-900 (2013). Read more (PubMed: 23493311) »

    See all 2 Publications for this product

    Product Wall



    This antibody was raised using a synthetic peptide corresponding to the C terminal end of the protein DHFR.

    Thank you for contacting Abcam regarding ab124814.


    For this product we do not have any information regarding storage at 4C. In general we would expect decreased activity over time when stored at this temperature. Furthermore, we do not ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"