Anti-Dihydrofolate reductase (DHFR) antibody, prediluted (ab82166)

Overview

  • Product name
    Anti-Dihydrofolate reductase (DHFR) antibody, prediluted
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description
    Rabbit polyclonal to Dihydrofolate reductase (DHFR), prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Dihydrofolate reductase (DHFR) (internal sequence).

  • Positive control
    • Breast Carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab82166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready to use. Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Target

  • Function
    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • Pathway
    Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
  • Sequence similarities
    Belongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHFR antibody
    • DHFRP1 antibody
    • Dihydrofolate reductase antibody
    • DYR antibody
    • DYR_HUMAN antibody
    • EC 1.5.1.3 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with Anti-DHFR antibody. Incubate primary antibody (ab82166) for 30 minutes at RT.

References

ab82166 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

I have reviewed the immunogen sequences of the DHFR antibodies we have and regrettably the alignment with the e coli amino acid sequence is very low. Therefore, we would not anticipate that these antibodies would ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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