Anti-Dihydropyrimidinase antibody (ab121844)

Overview

  • Product name
    Anti-Dihydropyrimidinase antibody
    See all Dihydropyrimidinase primary antibodies
  • Description
    Rabbit polyclonal to Dihydropyrimidinase
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TISRGKVVYE AGVFSVTAGD GKFIPRKPFA EYIYKRIKQR DRTCTPTPVE RAPYKGEVAT LKSRVTKEDA TAG, corresponding to amino acids 440-512 of Human Dihydropyrimidinase. (Q14117)

  • Positive control
    • Human kidney tissue; Dihydropyrimidinase over-expression HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
  • Tissue specificity
    Liver and kidney.
  • Involvement in disease
    Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD) [MIM:222748]. DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy.
  • Sequence similarities
    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications
    Carbamylation allows a single lysine to coordinate two zinc ions.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHP antibody
    • DHPase antibody
    • Dihydropyrimidinase antibody
    • Dihydropyrimidine amidohydrolase antibody
    • Dpys antibody
    • DPYS_HUMAN antibody
    • Hydantoinase antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles and centrosome. Recommended concentration of ab121844 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121844 at 1/500 dilution staining Dihydropyrimidinase in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • All lanes : Anti-Dihydropyrimidinase antibody (ab121844) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique

References

ab121844 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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