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Anti-dimethyl Arginine antibody [21C7] - ChIP Grade (ab413)

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Overview

Product name

Anti-dimethyl Arginine antibody [21C7] - ChIP Grade

Description

Mouse monoclonal [21C7] to dimethyl Arginine - ChIP Grade

Specificity

This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.

Tested applications

IP, WB, ChIPmore details

Immunogen

Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Raw Ascites

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

Clonality

Monoclonal

Clone number

21C7

Isotype

IgM

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H4 >> Methylated
Epigenetics and Nuclear Signaling >> Histones >> H2A >> Methylated
Signal Transduction >> Metabolism >> Amino Acids
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Methylated

Applications

Show applications key

Our Abpromise guarantee covers the use of ab413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Alternative names

  • Di methyl Arginine antibody

References for Anti-dimethyl Arginine antibody [21C7] - ChIP Grade (ab413)

This product has been referenced in:

  • Souki SK  et al. Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication. J Virol 83:5309-20 (2009). WB.Read more (PubMed: 19321610) »
  • Dolzhanskaya N  et al. Alternative splicing modulates protein arginine methyltransferase-dependent methylation of fragile X syndrome mental retardation protein. Biochemistry 45:10385-93 (2006).Read more (PubMed: 16922515) »

See all 5 publications for this product

Publishing research using ab413? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"