Anti-dimethyl Arginine antibody [21C7] (ab413)

Overview

  • Product name
    Anti-dimethyl Arginine antibody [21C7]
  • Description
    Mouse monoclonal [21C7] to dimethyl Arginine
  • Specificity
    This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
  • Tested applications
    Suitable for: IP, WB, ChIPmore details
  • Immunogen

    Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

  • General notes

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

Properties

Applications

Our Abpromise guarantee covers the use of ab413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/50 - 1/500.
ChIP Use at an assay dependent concentration. Use 5µl for 4x106 cells.

Target

  • Alternative names
    • Di methyl Arginine antibody

References

This product has been referenced in:

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

I can confirm that ab412 mono and dimethyl Arginine antibody [7E6] cross reacts with both symetric and assymetric methyl arginine. However, the ab413dimethyl Arginineantibodyis specific to ADMA only.

ht...

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Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor th...

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Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.
Username

Dr. Balint Laszlo Balint

Verified customer

Submitted Oct 04 2005

Thank you for your enquiry. Thank you also for completing our questionnaire so comprehensively. I am sorry that you have been having difficulties with our antisera against methyl arginine. I have examined your questionnaire and it seems as if you a...

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Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

Thank you for your enquiry and your interest in our products. We are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified. This antibody is provided as whole serum and the IgG concent...

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This antibody has not been tested in ELISA. All the latest information that we have about this antibody is found on the datasheet.

The epitope is di-methyl arginine independent of the surrounding sequence and has been used on numerous different diMeArg peptides.

Sorry, but the immunogen of this antibody is confidential. All we can say is that it contained methylated arginine (the antibody is inhibited by the modified amino acid).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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