Overview

  • Product nameAnti-Dinitrophenol antibody
    See all Dinitrophenol primary antibodies
  • Description
    Rabbit polyclonal to Dinitrophenol
  • SpecificityThe product is determined to be reactive with DNP-BSA and DNP-RSA but not BSA (bovine serum albumin) or RSA (rabbit serum albumin) by immunoelectrophoresis.
  • Tested applicationsSuitable for: ELISA, Functional Studies, WBmore details
  • Immunogen

    DNP conjugated to BSA.

  • General notes100 µl contains between 100 and 170 µg of specific antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab6306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • RelevanceDinitrophenol is an uncoupler, it has the ability to separate the flow of electrons and the pumping of H+ ions for ATP synthesis. This means that the energy from electron transfer cannot be used for ATP synthesis. 50 years ago, DNP was given as a drug to help patients lose weight.
  • Alternative names
    • DNP antibody

References for Anti-Dinitrophenol antibody (ab6306)

This product has been referenced in:
  • Kwolek-Mirek M  et al. Acrolein-Induced Oxidative Stress and Cell Death Exhibiting Features of Apoptosis in the Yeast Saccharomyces cerevisiae Deficient in SOD1. Cell Biochem Biophys N/A:N/A (2014). Read more (PubMed: 25395196) »
  • Orlandi I  et al. Sir2-dependent asymmetric segregation of damaged proteins in ubp10 null mutants is independent of genomic silencing. Biochim Biophys Acta 1803:630-638 (2010). Read more (PubMed: 20211662) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

We do have a rabbit polyclonal to Dinitrophenol as ab6306. I'd recommend that you look at the references cited on the online datasheet and below to make sure this antibody is suitable for your experiment as this a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5
Username

Maurizio Bettiga

Verified customer

Submitted Oct 28 2005

We would expect this antibody to be neutralized (bound) by these small compounds. They mimic DNP-protein.

To our knowledge, this anitbody has not yet been tested in this application

No, I'm afraid that all the information we have for this product is on the datasheet.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"