Overview

  • Product nameAnti-DIO2 antibody
    See all DIO2 primary antibodies
  • Description
    Rabbit polyclonal to DIO2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Goat, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 DIO2.

    (Peptide available as ab87369.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Human Heart and Placenta, Mouse retina, P0 Whole Brain and Rat Whole Brain. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-DIO2 antibody images

  • ICC/IF image of ab77779 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77779 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 105 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-DIO2 antibody (ab77779)

ab77779 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions Reduced Denaturing
Loading amount 100 µg
Treatment pCMX-DIO2 CDS transfection
Specification HEK293T
Blocking step Blocking one(Nacarai Tesque) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

一郎 山内

Verified customer

Submitted Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"