The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Deiodinase, iodothyronine, type II
Thyroxine deiodinase, type II
Type 2 DI
Type 2 iodothyronine deiodinase
Type II 5' deiodinase
Type II iodothyronine deiodinase
FunctionResponsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Tissue specificityHeart, skeletal muscle, placenta, fetal brain and several regions of the adult brain.
Sequence similaritiesBelongs to the iodothyronine deiodinase family.
Post-translational modificationsUbiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.