Overview

  • Product name
    Anti-Dio3 antibody
  • Description
    Rabbit polyclonal to Dio3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within the C terminal amino acids 249-278 of Human Dio3 (NP_001353).

  • Positive control
    • HepG2 cell line lysates. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab102926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
  • Tissue specificity
    Expressed in placenta and several fetal tissues.
  • Sequence similarities
    Belongs to the iodothyronine deiodinase family.
  • Cellular localization
    Cell membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5DIII antibody
    • D3 antibody
    • Deiodinase iodothyronine type 3 antibody
    • Deiodinase iodothyronine type III antibody
    • Dio 3 antibody
    • Dio3 antibody
    • DIOIII antibody
    • IOD3_HUMAN antibody
    • Iodothyronine deiodinase placental type antibody
    • MGC124117 antibody
    • MGC124118 antibody
    • Thyroxine deiodinase type III antibody
    • Thyroxine deiodinase type III (selenoprotein) antibody
    • TXDI3 antibody
    • Type 3 DI antibody
    • Type 3 iodothyronine selenodeiodinase antibody
    • Type III 5' deiodinase antibody
    • Type III iodothyronine deiodinase antibody
    • Type-III 5''-deiodinase antibody
    see all

Images

  • Anti-Dio3 antibody (ab102926) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 34 kDa
  • ab102926 at 1/50 staining Dio3 in Formalin-fixed and paraffin-embedded Human hepatocarcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

References

This product has been referenced in:
  • Kagami M  et al. Paternal uniparental disomy 14 and related disorders: placental gene expression analyses and histological examinations. Epigenetics 7:1142-50 (2012). Read more (PubMed: 22917972) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (aortic endothelial cells)
Permeabilization
Yes - Saponin 0,1%
Specification
aortic endothelial cells
Blocking step
PBS/Saponin 0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
PFA 4%
Username

Abcam user community

Verified customer

Submitted Jul 08 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - other (Heart)
Specification
Heart
Blocking step
Super Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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