Anti-DIP13B antibody - C-terminal (ab154661)

Overview

  • Product name
    Anti-DIP13B antibody - C-terminal
    See all DIP13B primary antibodies
  • Description
    Rabbit polyclonal to DIP13B - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within C terminal amino acids 476 and 664 of Human DIP13B (Uniprot ID: Q8NEU8)

  • Positive control
    • NT2D1 or IMR32 whole cell lysate; A431 cells; HBL435 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.
  • Tissue specificity
    High levels in brain, heart, kidney and skeletal muscle.
  • Involvement in disease
    Note=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2.
  • Sequence similarities
    Contains 1 PH domain.
    Contains 1 PID domain.
  • Cellular localization
    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter protein containing PH domain antibody
    • Adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody
    • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 antibody
    • APLP2 antibody
    • Appl2 antibody
    • DCC interacting protein 13 beta antibody
    • DCC-interacting protein 13-beta antibody
    • Dip13 beta antibody
    • Dip13-beta antibody
    • DP13B_HUMAN antibody
    • PTB domain and leucine zipper motif 2 antibody
    see all

Images

  • All lanes : Anti-DIP13B antibody - C-terminal (ab154661) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling DIP13B using ab154661 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling DIP13B using ab154661 at 1/100 dilution.

References

ab154661 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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