Anti-Diphtheria Toxin A subunit antibody [7F2] (ab8310)

Overview

  • Product nameAnti-Diphtheria Toxin A subunit antibody [7F2]
    See all Diphtheria Toxin A subunit primary antibodies
  • Description
    Mouse monoclonal [7F2] to Diphtheria Toxin A subunit
  • SpecificityThis antibody does not react with the free B subunit of Diphtheria toxin.
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Full length protein.

  • EpitopeThis antibody reacts with the epitope exposed on the free A subunit and on the whole Diphtheria toxin molecule.
  • General notes

    Detection of Diphtheria toxin A subunit

    For a recombinant antibody to Diphtheria Toxin, please see - Ab209329

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [8A4] to Diphtheria Toxin A subunit (ab8308). Use as detection antibody.

Target

  • RelevanceDiphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
  • Alternative names
    • Diphtheria Toxin antibody

References for Anti-Diphtheria Toxin A subunit antibody [7F2] (ab8310)

This product has been referenced in:
  • Buch T  et al. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2:419-26 (2005). Read more (PubMed: 15908920) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Escherichia coli Recombinant protein (supernatant)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification supernatant
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jan 30 2016

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Dear Masanori ab8310 - This antibody reacts with the epitope exposed on the free A subunit and on the whole Diphtheria toxin molecule. ab8308 - this has not been fully characterised. This is currently the only information that we have.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"