Anti-Diphtheria Toxin A subunit antibody [8A4] (ab8308)

Overview

  • Product nameAnti-Diphtheria Toxin A subunit antibody [8A4]
    See all Diphtheria Toxin A subunit primary antibodies
  • Description
    Mouse monoclonal [8A4] to Diphtheria Toxin A subunit
  • SpecificityThis antibody reacts with free A subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin or free B subunit of Diphtheria toxin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Immunogen

    Full length protein.

  • General notes

    Detection of Diphtheria toxin A subunit

    For a recombinant antibody to Diphtheria Toxin, please see - Ab209329

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [7F2] to Diphtheria Toxin A subunit (ab8310). Use as capture antibody.
WB Use at an assay dependent concentration.

Target

  • RelevanceDiphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
  • Alternative names
    • Diphtheria Toxin antibody

References for Anti-Diphtheria Toxin A subunit antibody [8A4] (ab8308)

ab8308 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 350 µg
Gel Running Conditions Reduced Denaturing
Sample African Green Monkey Cell lysate - whole cell (Kidney Epithelial (vero))
Specification Kidney Epithelial (vero)
Blocking step Licor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Amy

Verified customer

Submitted May 31 2013

The epitopes have not been fully mapped for either of the two antibodies. We do not have a specific ELISA protocol for these and suggest customers use the general protocol on the Abcam homepage.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"